Using ABI3100, I sometimes encountered bad electrophoresis where bands migration delayed in later part of electrophoresis. Bad electrophoresis is more frequent just after polymer or capillary change. This might be because of incomplete debubbling, and after a few runs it would become OK, or in some other cases one or two particular capillaries among 16 would be stubborn.
A sequence sample of bad electrophoresis on ABI 3100.
It wastes time to open, scroll and see all the sequence files to check for ABI3100. It is convenient and economy of time for ABI3100 to check the run history. Select Run History menu of the UDC and click on Find All to show a list of runs. Select a particular run to check. Click on Cap/Array Viewer menu to show the gel image. Bad electrophoresed capillaries can easily be recognizable.
A run history of ABI 3100. Capillaries #9 (well #E03) and #10 (well #E04) show delayed migration of bands.
Transfer samples of bad electrophoresis to a new PCR plate to set onto the machine, make another sample sheet to fit the new sample layout, and rerun. ABI3100 can run samples a few times without loss of signals. It is unnecessary to make sequence reaction again to rerun with ABI3100.
