0.
Start Here;
to use Amersham ET Kit
on ABI 3100(Win 2000 Version)
1. Economy Sequencing Reaction
2. High Throughput Ethanol Precipitation
of Sequencing Reaction Product
3. Electrophoresis Buffer
of 13-Times Economy
4. Electrophoresis Buffer
of 8-Times Economy
5. Loading Dye of 3-Times Economy
6. Electrophoresis Polymer (POP6)
of 26-Times Economy
7. Replacement of Long Read Polymer
for 26-Times Economy (POP5 Long Read Protocol)
8. Use of Long Read Polymer
for 5-Times Economy (POP7 Long Read Protocol)
9. Use of EXCEL to make a shortcut
to make plate data
Tips shown here are tested on an ABI3100 (Win2000 version) which is controled by UDC V.2.0 and SeqA V.5.1~5.1.1. Operation procedures listed above may be specific to this version of machine and different from earlier WinNT version. Moreover, there may be some minor differences from machine to machine. The author, therefore, do not give any warranty for use of above technical tips.