C O N T E N T S --------------------------------------- 1. IUB nucleotide code table 2. Codon tables 2a. Universal 2b. Vertebrate mitochondrial 3. Amino acid abbreviations 4. Plasmid multi-cloning-sites 4a. pBluescript2 4aI KS-forward 4aII KS-reverse 4aIII SK-forward 4aIV SK-reverse 4b. pTAC-2 4cI pTAC-2-forward 4cII pTAC-2-reverse 4c. pGEM-t 4cI pGEM-t-forward 4cII pGEM-t-reverse 4d. M13/pUC 4bI 19-forward 4bII 19-reverse 4bIII 18-forward 4bIV 18-reverse 5. Lambda DNA markers 6. Restriction enzymes --------------------------------------- IUB nucleotide code table ------------------------------------------- Code Complement ------------------------------------------- A Adenine T C Cytocine G G Guanine C T Thymine A U Uracil A R A/G puRine Y Y C/T/U pYrimidine R N A/G/C/T/U Nucleotides N S C/G Strong S W A/T/U Weak W K G/T/U Keto M M A/C aMino K B C/G/T/U not A V V A/C/G not T/U B D A/G/T/U not C H H A/C/T/U not G D ------------------------------------------- Universal Codon Table TTT Phe F TCT Ser S TAT Tyr Y TGT Cys C TTC Phe F TCC Ser S TAC Tyr Y TGC Cys C TTA Leu L TCA Ser S TAA *(ochre) TGA *(umber) TTG Leu L TCG Ser S TAG *(amber) TGG Trp W CTT Leu L CCT Pro P CAT His H CGT Arg R CTC Leu L CCC Pro P CAC His H CGC Arg R CTA Leu L CCA Pro P CAA Gln Q CGA Arg R CTG Leu L CCG Pro P CAG Gln Q CGG Arg R ATT Ile I ACT Thr T AAT Asn N AGT Ser S ATC Ile I ACC Thr T AAC Asn N AGC Ser S ATA Ile I ACA Thr T AAA Lys K AGA Arg R ATG* Met M ACG Thr T AAG Lys K AGG Arg R GTT Val V GCT Ala A GAT Asp D GGT Gly G GTC Val V GCC Ala A GAC Asp D GGC Gly G GTA Val V GCA Ala A GAA Glu E GGA Gly G GTG* Val V GCG Aia A GAG Glu E GGG Gly G Vertebrate Mitochondrial Codon Table TTT Phe F TCT Ser S TAT Tyr Y TGT Cys C TTC Phe F TCC Ser S TAC Tyr Y TGC Cys C TTA Leu L TCA Ser S TAA * TGA Trp W TTG Leu L TCG Ser S TAG * TGG Trp W CTT Leu L CCT Pro P CAT His H CGT Arg R CTC Leu L CCC Pro P CAC His H CGC Arg R CTA Leu L CCA Pro P CAA Gln Q CGA Arg R CTG Leu L CCG Pro P CAG Gln Q CGG Arg R ATT Ile I ACT Thr T AAT Asn N AGT Ser S ATC* Ile I ACC Thr T AAC Asn N AGC Ser S ATA* Met M ACA Thr T AAA Lys K AGA * ATG* Met M ACG Thr T AAG Lys K AGG * GTT Val V GCT Ala A GAT Asp D GGT Gly G GTC Val V GCC Ala A GAC Asp D GGC Gly G GTA Val V GCA Ala A GAA Glu E GGA Gly G GTG* Val V GCG Aia A GAG Glu E GGG Gly G Amino Acid Abbreviations ------------------------ Alanine Ala A Arginine Arg R Asparagine Asn N Aspartate Asp D Cysteine Cys C Glutamate Glu E Glutamine Gln Q Glycine Gly G Histidine His H Isoleucine Ile I Leucine Leu L Lysine Lys K Methionine Met M Phenilalanine Phe F Proline Pro P Serine Ser S Threonine Thr T Tryptophan Trp W Tyrosine Tyr Y Valine Val V ------------------------ KS-MCS-F 5'protrusive XhoI, AccI, SalI, ClaI(BanIII), HindIII, EcoRI, BamHI, SpeI, XbaI, NotI 3'protrusive (x); unuseful for ExoIII deletion. KpnI, ApaI, PstI, SacII(x), BstXI, SacI blunt HincII, EcoRV, SmaI ----+----1----+----2----+----3----+----4----+----5 CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGC --------M13FW.HT-------> -----M13-21------> BssHII GTAATACGACTCACTATAGGGCGAATTGGAGCTCCACCGCGGTGGCGGCCGC --------T7-----------> SacI SacII NotI <- BstXI -> --- TCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGATATCAAGCTT XbaI SpeI BamHI SmaI PstI EcoRI EcoRV Hind3 ------SK--------> ATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTT ClaI SalI XhoI ApaI KpnI <--------KS------- CCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGT <----------T3-------BssHII <-------RV-- <- TTCCTGTGTGAAATTGTTATCCGCT ------ --------M13RV.HT--------- KS-MCS-R 5'protrusive XhoI, AccI, SalI, ClaI(BanIII), HindIII, EcoRI, BamHI, SpeI, XbaI, NotI 3'protrusive (x); unuseful for ExoIII deletion. KpnI, ApaI, PstI, SacII(x), BstXI, SacI blunt HincII, EcoRV, SmaI ----+----1----+----2----+----3----+----4----+----5 AGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAA --------RV-------> ---------M13RV.HT---------> GCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCGGGCCCC BssHII-------T3----------> KpnI ApaI CCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGG XhoI SalI ClaI Hind3 EcoRV EcoRI PstI SmaI --------KS-------> GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATT BamHI SpeI XbaI NotI SacII SacI <--------SK--------- <- BstXI -> CGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAA <---------T7----------BssHII <------M13-21----- CGTCGTGACTGGGAAAACCCTGGCG <--------M13FW.HT------- SK-MCS-F 5'protrusive NotI, XbaI, SpeI, BamHI, EcoRI, HindIII, ClaI(BanIII), AccI, SalI, XhoI 3'protrusive (x); unuseful for ExoIII deletion. SacI, BstXI, SacII(x), PstI, ApaI, KpnI blunt SmaI, EcoRV, HincII ----+----1----+----2----+----3----+----4----+----5 CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGC -------M13FW.HT--------> -----M13-21------> BssHII GTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAG --------T7-----------> KpnI ApaI XhoI ----- GTCGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCC SalI ClaI Hind3 EcoRV EcoRI PstI SmaI BamHI ----KS------> <---- ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTT SpeI XbaI NotI SacII SacI ----SK--------- <- BstXI -> CCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGT <--------T3---------BssHII <-------RV-- <- TTCCTGTGTGAAATTGTTATCCGCT ------ --------M13RV.HT--------- SK-MCS-R 5'protrusive NotI, XbaI, SpeI, BamHI, EcoRI, HindIII, ClaI(BanIII), AccI, SalI, XhoI 3'protrusive (x); unuseful for ExoIII deletion. SacI, BstXI, SacII(x), PstI, ApaI, KpnI blunt SmaI, EcoRV, HincII ----+----1----+----2----+----3----+----4----+----5 AGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAA --------RV-------> ---------M13RV.HT---------> GCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGG BssHII SacI SacII -------T3----------> <- BstXI TGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGATATC NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV -> -------SK----------> AAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAATT Hind3 ClaI SalI XhoI ApaI KpnI <---------KS------ CGCCCTATAGTGAGTCGTA TTACGCGCGCTCACTGGCCGTCGTTTTACA <---------T7----------BssHII <------M13-21----- ACGTCGTGACTGGGAAAACCCTGGCG <--------M13FW.HT------- pTAC-2 MCS-F 5' protrusive (x); useful for excision of insert with MCS NotI(x), EcoRI, XhoI, BamHI, XbaI, SalI, HindIII, NotI(x) 3' protrusive SacI, KpnI, PstI, SphI blunt SmaI Enzymes that do not cut (x); a site appear at TA cloning site after ligation ApaI, BclI, BglII(x), BssHII, BstXI, ClaI, DraIII, EcoRV, HpaI, NcoI, SacII, SpeI, StuI, StyI ----+----1----+----2----+----3----+----4----+----5 CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCT --------M13FW.HT-------> -----M13-21------> AGTGTAATACGACTCACTATAGGGCGCGGCCGCAGAATTCGAGCTCGGTA --------T7----------->NotI EcoRI SacI KpnI CCCGGGATCTCGAGGCCAGATCt^aATTGTGGATCCGCTCTAGAGTCGAC SmaI XhoI (BglII) BamHI XbaI SalI CTGCAGGCATGCAAGCTTGCGGCCGCGTATTCTATAGTGTCACCTAAATAGC PstI SphI Hind3 NotI <-------SP6-------- ATGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT <-------RV-------- <-----------M13RV.HT------- ^ TA cloning site is not at 453 but 473. pTAC-2 MCS-R 5' protrusive (x); useful for excision of insert with MCS NotI(x), HindIII, SalI, XbaI, BamHI, XhoI, EcoRI, NotI(x) 3' protrusive SphI, PstI, KpnI, SacI blunt SmaI Enzymes that do not cut (x); a site appear at TA cloning site after ligation ApaI, BclI, BglII(x), BssHII, BstXI, ClaI, DraIII, EcoRV, HpaI, NcoI, SacII, SpeI, StuI, StyI ----+----1----+----2----+----3----+----4----+----5 AGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAT -----------M13RV.HT-------> -------RV--------> GCTATTTAGGTGACACTATAGAATACGCGGCCGCAAGCTTGCATGCCTGCAG --------SP6-------> NotI Hind3 SphI PstI GTCGACTCTAGAGCGGATCCACAATt^aGATCTGGCCTCGAGATCCCG SalI XbaI BamHI (BglII) XhoI SmaI GGTACCGAGCTCGAATTCTGCGGCCGCGCCCTATAGTGAGTCGTATTACAC KpnI SacI EcoRI NotI<-----------T7------------ TAGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCG <-----M13-21------ <-------M13FW.HT-------- ^ TA cloning site is not at 453 but 473. pGEM-t-MCS-F 5'protrusive BstZI, NcoI, SpeI, NotI, SalI, NdeI 3'protrusive (x); unuseful for ExoIII deletion. ApaI, AatII, SphI, SacII(x), PstI, SacI, BstXI, NsiI t^a, linealized and T added site. ----+----1----+----2----+----3----+----4----+----5 CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATT --------M13FW.HT-------> -----M13-21------> GTAATACGACTCACTATAGGGCGAATTGGGCCCGACGTCGCATGCTCCCGGCCG --------T7-----------> ApaI AatII SphI BstZI CCATGGCCGCGGGATt^aATCACTAGTGCGGCCGCCTGCAGGTCGAC NcoI SacII SpeI NotI PstI SalI BstZI CATATGGGAGAGCTCCCAACGCGTTGGATGCATAGCTTGAGTATTCTATAG NdeI SacI <- BstXI ->NsiI <----- TGTCACCTAAATAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTG ----SP6------ <-------RV-------- <----------- AAATTGTTATCCGCT M13RV.HT------- pGEM-t-MCS-R 5'protrusive NdeI, SalI, NotI, SpeI, NcoI, BstZI 3'protrusive (x); unuseful for ExoIII deletion. NsiI, BstXI, SacI, PstI, SacII(x), SphI, AatII, ApaI t^a, linealized and T added site. ----+----1----+----2----+----3----+----4----+----5 AGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAA ---------M13RV.HT---------> --------RV-------> GCTATTTAGGTGACACTATAGAATACTCAAGCTATGCATCCAACGCGTTGG -------SP6--------> NsiI <- BstXI -> GAGCTCTCCCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATt^aATC SacI NdeI SalI PstI NotI SpeI BstZI CCGCGGCCATGGCGGCCGGGAGCATGCGACGTCGGGCCCAATTCGCCCT SacII NcoI BstZI SphI AatII ApaI <---- ATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGAC ------T7--------- <-----M13-21------ <------- TGGGAAAACCCTGGCG ----M13FW.HT---- pUC19-MCS-F 5'protrusive EcoRI, BamHI, XbaI, SalI, AccI, HindIII 3'protrusive SacI, KpnI, PstI, SphI blunt SmaI, HincII ----+----1----+----2----+----3----+----4----+----5 CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTC --------M13FW.HT-------> -----M13-21------>EcoRI GAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTT SacI KpnISmaI BamHI XbaI SalI PstI SphI Hind3 AccI HincII GGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT <--------RV------- <---------M13RV.HT--------- pUC19-MCS-R 5'protrusive EcoRI, BamHI, XbaI, SalI, AccI, HindIII 3'protrusive SacI, KpnI, PstI, SphI blunt SmaI, HincII ----+----1----+----2----+----3----+----4----+----5 AGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTT -------RV--------> Hind3 ---------M13RV.HT---------> GCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTC SphI PstI SalI XbaI BamHISmaIKpnI SacI AccI HincII GAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCG EcoRI <-----M13-21------ <--------M13FW.HT------- pUC18-MCS-F 5'protrusive EcoRI, BamHI, XbaI, SalI, AccI, HindIII 3'protrusive SacI, KpnI, PstI, SphI blunt SmaI, HincII ----+----1----+----2----+----3----+----4----+----5 CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTT -------M13FW.HT--------> -----M13-21------> Hind3 GCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTC SphI PstI SalI XbaI BamHISmaIKpnI SacI EcoRI AccI HincII GTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT <--------RV------- <---------M13RV.HT--------- pUC18-MCS-R 5'protrusive EcoRI, BamHI, XbaI, SalI, AccI, HindIII 3'protrusive SacI, KpnI, PstI, SphI blunt SmaI, HincII ----+----1----+----2----+----3----+----4----+----5 AGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTC --------RV-------> EcoRI ---------M13RV.HT---------> GAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTT SacI KpnISmaI BamHI XbaI SalI PstI SphI Hind3 AccI HincII GGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCG <-----M13-21------ <-------M13FW.HT-------- Fragment Size and %DNA Amount of Lambda DNA Markers Lambda-HindIII Digest Lambda-EcoT14I Digest fragment # length(bp) % amount ng of 250ng fragment # length(bp) % amount ng of 250ng 1 23130* 47.7 119.2 1 19329* 39.9 99.6 2 9416 19.4 48.5 2 7743 16.0 39.9 3 6557 13.5 33.8 3 6223 12.8 32.1 4 4361* 9.0 22.5 4 4254* 8.8 21.9 5 2322 4.8 12.0 5 3472 7.2 17.9 6 2027 4.2 10.4 6 2690 5.5 13.9 7 564 1.2 2.9 7 1882 3.9 9.7 8 125 0.3 0.6 8 1489 3.1 7.7 9 925 1.9 4.8 10 421 0.9 2.2 11 74 0.2 0.4 * Fragments sticking to each other at the cos site, band intensity on a gel may be different from what is expected from their lengths. Restriction Enzyme Table ---------------------------------------------------- enzyme sequence cut point (!) ---------------------------------------------------- AatI AGGCCT AGG!CCT AatII GACGTC GACGT!C AccI GTMKAC GT!MKAC AccII CGCG CG!CG AccIII TCCGGA T!CCGGA AcyI GRCGYC GR!CGYC AflI GGWCC G!GWCC AflII CTTAAG C!TTAAG AflIII ACRYGT A!CRYGT AhaI CCSGG CC!SGG AhaII GRCGYC GR!CGYC AhaIII TTTAAA TTT!AAA AluI AGCT AG!CT AlwI GGATC GGATCNNNN! AlwI GATCC !NNNNNGATCC AlwNI CAGNNNCTG CAGNNN!CTG AocI CCTNAGG CC!TNAGG AocII GDGCHC GDGCH!C AosI TGCGCA TGC!GCA AosII GRCGYC GR!CGYC ApaI GGGCCC GGGCC!C ApaLI GTGCAC G!TGCAC ApyI CCWGG CC!WGG AquI CYCGRG C!YCGRG AseI ATTAAT AT!TAAT Asp700I GAANNNNTTC GAANN!NNTTC Asp718I GGTACC G!GTACC AspI GACNNNGTC GACN!NNGTC AsuI GGNCC G!GNCC AsuII TTCGAA TT!CGAA AvaI CYCGRG C!YCGRG AvaII GGWCC G!GWCC AvaIII ATGCAT ATGCA!T AvrI CYCGRG !CYCGRG AvrII CCTAGG C!CTAGG AxyI CCTNAGG CC!TNAGG BalI TGGCCA TGG!CCA BamHI GGATCC G!GATCC BanI GGYRCC G!GYRCC BanII GRGCYC GRGCY!C BanIII ATCGAT AT!CGAT BbeI GGCGCC GGCGC!C BbiII/AcyI GRCGYC GR!CGYC BbvI GCAGC GCAGCNNNNNNNN! BbvI GCTGC !NNNNNNNNNNNNGCTGC BbvII GAAGAC GAAGACNN! BbvII GTCTTC !NNNNNNGTCTTC BcefI ACGGC ACGGCNNNNNNNNNNNN! BcefI GCCGT !NNNNNNNNNNNNNGCCGT BclI TGATCA T!GATCA BcnI CCSGG CC!SGG BglI GCCNNNNNGGC GCCNNNN!NGGC BglII AGATCT A!GATCT BinI GATCC !NNNNNGATCC BinI GGATC GGATCNNNN! BsePI GCGCGC !GCGCGC BsmAI GTCTC !GTCTC BsmAI GAGAC !GAGAC BsmI GAATGC GAATGCN! BsmI GCATTC G!CATTC Bsp1286I GDGCHC GDGCH!C BspHI TCATGA T!CATGA BspMI ACCTGC ACCTGCNNNN! BspMI GCAGGT !NNNNNNNNGCAGGT BspMII TCCGGA T!CCGGA BsrI ACTGG ACTGGN! BsrI CCAGT C!CAGT BssHII GCGCGC G!CGCGC BstBI TTCGAA TT!CGAA BstEII GGTNACC G!GTNACC BstI GGATCC G!GATCC BstNI CCWGG CC!WGG BstPI GGTNACC G!GTNACC BstUI CGCG CG!CG BstXI CCANNNNNNTGG CCANNNNN!NTGG BstYI RGATCY R!GATCY Bsu36I CCTNAGG CC!TNAGG CcrI CTCGAG C!TCGAG CfoI GCGC GCG!C Cfr10I RCCGGY R!CCGGY Cfr13I GGNCC G!GNCC CfrI YGGCCR Y!GGCCR ClaI ATCGAT AT!CGAT CviJI RGCY RG!CY CvnI CCTNAGG CC!TNAGG DdeI CTNAG C!TNAG DpnI GATC GA!TC DraI TTTAAA TTT!AAA DraII RGGNCCY RG!GNCCY DraIII CACNNNGTG CACNNN!GTG DsaI CCRYGG C!CRYGG EaeI YGGCCR Y!GGCCR EagI CGGCCG C!GGCCG EarI CTCTTC !CTCTTC EarI GAAGAG !GAAGAG EclXI CGGCCG C!GGCCG Eco105I TACGTA TAC!GTA Eco31I GGTCTC GGTCTCN! Eco31I GAGACC !NNNNNGAGACC Eco47I GGWCC G!GWCC Eco47III AGCGCT AGC!GCT Eco52I CGGCCG C!GGCCG Eco57I CTGAAG !CTGAAG Eco57I CTTCAG !CTTCAG Eco81I CCTNAGG CC!TNAGG EcoNI CCTNNNNNAGG CCTNN!NNNAGG EcoO109I RGGNCCY RG!GNCCY EcoRI GAATTC G!AATTC EcoRII CCWGG !CCWGG EcoRV GATATC GAT!ATC EcoT14I CCWWGG C!CWWGG EcoT22I ATGCAT ATGCA!T EcoT38I GRGCYC GRGCY!C EheI GGCGCC GGC!GCC EspI GCTNAGC GC!TNAGC FinI GTCCC !GTCCC FinI GGGAC !GGGAC Fnu4HI GCNGC GC!NGC FokI GGATG GGATGNNNNNNNNN! FokI CATCC !NNNNNNNNNNNNNCATCC FspI TGCGCA TGC!GCA GdiII YGGCCG !NNNNNYGGCCG GdiII CGGCCR CGGCCRN! GsuI CTCCAG !CTCCAG GsuI CTGGAG !CTGGAG HaeI WGGCCW WGG!CCW HaeII RGCGCY RGCGC!Y HaeIII GGCC GG!CC HapII CCGG C!CGG HgaI GACGC GACGCNNNNN! HgaI GCGTC !NNNNNNNNNNGCGTC HgiAI GWGCWC GWGCW!C HgiEII ACCNNNNNNGGT !ACCNNNNNNGGT HhaI GCGC GCG!C Hin1I GRCGYC GR!CGYC HinP1I GCGC G!CGC HincII GTYRAC GTY!RAC HindIII AAGCTT A!AGCTT HinfI GANTC G!ANTC HpaI GTTAAC GTT!AAC HpaII CCGG C!CGG HphI GGTGA GGTGANNNNNNNN! HphI TCACC !NNNNNNNTCACC KpnI GGTACC GGTAC!C Ksp632I CTCTTC CTCTTCN! Ksp632I GAAGAG !NNNNGAAGAG MaeI CTAG C!TAG MaeII ACGT A!CGT MaeIII GTNAC !GTNAC MboI GATC !GATC MboII GAAGA GAAGANNNNNNNN! MboII TCTTC !NNNNNNNTCTTC MfeI CAATTG !CAATTG MflI RGATCY R!GATCY MluI ACGCGT A!CGCGT MmeI TCCRAC !TCCRAC MmeI GTYGGA !GTYGGA MnlI CCTC CCTCNNNNNNN! MnlI GAGG !NNNNNNNGAGG MroI TCCGGA T!CCGGA MseI TTAA T!TAA MspI CCGG C!CGG MstI TGCGCA TGC!GCA MstII CCTNAGG CC!TNAGG MvaI CCWGG CC!WGG NaeI GCCGGC GCC!GGC NarI GGCGCC GG!CGCC NciI CCSGG CC!SGG NcoI CCATGG C!CATGG NdeI CATATG CA!TATG NdeII GATC !GATC NheI GCTAGC G!CTAGC NlaIII CATG CATG! NlaIV GGNNCC GGN!NCC NotI GCGGCCGC GC!GGCCGC NruI TCGCGA TCG!CGA NsiI ATGCAT ATGCA!T Nsp(7524)I RCATGY RCATG!Y Nsp(7524)V TTCGAA TT!CGAA NspBII CMGCKG CMG!CKG NspII GDGCHC GDGCH!C NspIII CYCGRG C!YCGRG NspIV GGNCC G!GNCC NunII GGCGCC GG!CGCC PaeR71 CTCGAG C!TCGAG PalI GGCC GG!CC PflMI CCANNNNNTGG CCANNNN!NTGG PleI GAGTC GAGTCNNNN! PleI GACTC !NNNNNGACTC PmaCI CACGTG CAC!GTG PpuMI RGGWCCY RG!GWCCY PstI CTGCAG CTGCA!G PvuI CGATCG CGAT!CG PvuII CAGCTG CAG!CTG RsaI GTAC GT!AC RsrI GAATTC G!AATTC RsrII CGGWCCG CG!GWCCG SacI GAGCTC GAGCT!C SacII CCGCGG CCGC!GG SalI GTCGAC G!TCGAC Sau3AI GATC !GATC Sau96I GGNCC G!GNCC SauI CCTNAGG CC!TNAGG ScaI AGTACT AGT!ACT ScrFI CCNGG CC!NGG SduI GDGCHC GDGCH!C SecI CCNNGG C!CNNGG SexI CTCGAG !CTCGAG SfaNI GCATC GCATCNNNNN! SfaNI GATGC !NNNNNNNNNGATGC SfiI GGCCNNNNNGGCC GGCCNNNN!NGGCC SinI GGWCC G!GWCC SmaI CCCGGG CCC!GGG SnaBI TACGTA TAC!GTA SnaI GTATAC !GTATAC SpeI ACTAGT A!CTAGT SphI GCATGC GCATG!C SplI CGTACG C!GTACG SspI AATATT AAT!ATT SstI GAGCTC GAGCT!C SstII CCGCGG CCGC!GG SstIII ACGT !ACGT StuI AGGCCT AGG!CCT StyI CCWWGG C!CWWGG StySJI GAGNNNNNNGTRC !GAGNNNNNNGTRC StySJI GYACNNNNNNCTC !GYACNNNNNNCTC TaqI TCGA T!CGA TaqII GACCGA GACCGANNNNNNNNNNN! TaqII TCGGTC !NNNNNNNNNTCGGTC TaqII CACCCA CACCCANNNNNNNNNNN! TaqII TGGGTG !NNNNNNNNNTGGGTG ThaI CGCG CG!CG Tsp45I GTSAC !GTSAC TspEI AATT !AATT Tth111I GACNNNGTC GACN!NNGTC Tth111II CAARCA CAARCANNNNNNNNNNN! Tth111II TGYTTG !NNNNNNNNNTGYTTG TthHB8I TCGA T!CGA VspI ATTAAT AT!TAAT XbaI TCTAGA T!CTAGA XcyI CCCGGG C!CCGGG XhoI CTCGAG C!TCGAG XhoII RGATCY R!GATCY XmaI CCCGGG C!CCGGG XmaIII CGGCCG C!GGCCG XmnI GAANNNNTTC GAANN!NNTTC XorII CGATCG CGAT!CG ----------------------------------------------------