PARFEX (Parents Finder in Microsoft® EXCELTM) is a computer program for molecular parentage analysis. It is built in an EXCEL workbook (EXCEL macros), therefore a series of parentage testing completes in EXCEL. The Exclusion method and likelihood-ratio method are available in this software.
PARFEX runs in MS-Windows EXCEL.
It handles autosomal co-dominant microsatellite and SNP (single nucleotide polymorphism) markers. The program you may download below allows up to 120 markers for calculation. If you need to analyze more markers, please do not hesitate to ask me (see below for email).
Several accessory macros, which assist parentage testing, are bundled in. For example, one subprogram calculates summary statistics of polymorphisms (e.g., PIC, exclusion probabilities). Another subprogram generates simulated offspring and random individuals based on parental genotype data.
PARFEX (ver. 1.0) is available here (zipped): PARFEX with user guide
The zipped folder contains two EXCEL files. EXCEL 2003 users better use the file ‘PARFEX_v1_0_E03.xls’. For those who have EXCEL 2007 or more recent version, better use ‘PARFEX_v1_0.xlsm.’
If you find problems in downloading or aberrant behavior of PARFEX, please feel free to inquire of Dr Masashi Sekino (sekino, immediately followed by @affrc.go.jp) of National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency.
PARFEX was developed by Drs Masashi Sekino’s and Shigeho Kakehi’s joint effort. Please cite the following when you publish an article containing results obtained with PARFEX:
Sekino M, Kakehi S (2012) PARFEX v1.0: an EXCELTM-based software package for parentage allocation. Conservation Genetics Resources 4:275–278
Changes or bug-fix
4 Apr., 2018: In the program Exclusion, the output of single parent(s) was omitted when one parental pair or more were detected. This specification has been changed. Now all single parents and parental pairs come up in the result sheet. Many thanks to Dr. D. Noguchi for his request to do so.
29 Nov., 2012: A bug occurring in reading genotype data has been fixed.
13 Jun., 2012: In order to reduce computation time, we modified a subprogram ‘Lhood_PrvLOD’ used in likelihood-based method.
8 Dec., 2011: We added one option to a subprogram ‘PFX_Varstat’. By this user may omit HWE testing (Monte Carlo exact test). We provide this option since HWE analysis in PARFEX takes much computation time for a large sample size.